ESR2: Evolution of the small intestine microbiome in pigs from birth to post weaning under normal and stress conditions
Targeted manipulation of the gut microbiota is increasingly recognized as a mean to improve livestock health. We know that the interpretation of bacterial communities of the small intestine by studying the rectal microbiome from stool samples has its limitations. Thus, getting a more in depth understanding of the precise role of the intestinal microbiome in health and disease requires a method to access the ‘true’ (not fecal) intestinal microbiome.
- Set up and validate an in vivo sampling tool (e.g. capsule endoscopy) to collect small intestine content in living pigs.
- Determine to what extent the small intestine microbiome changes over time and to assess the temporal stability of an individual pig’s gut microbiome.
- Compare previous results with pathophysiologic conditions to evaluate the consequences of an ETEC infection, as a model for pathogens interference.
- In a first step, the new in vivo sampling tool will be optimized and validated.
- In subsequent steps, the microbiome development through pigs life cycle will be monitored under normal and pathophysiological conditions.
- Novel in vivo sampling tool for small intestine content will be validated and standard operating procedure prepared (D2.1).
- Extended knowledge on the evolution of phylotypes and/or bacterial species in the small intestine microbiome from post weaning to slaughter (D2.4) under physiological and pathophysiological conditions (D3.7).
- At: UNIBO (7 mo); Bioinformatics for microbiome and metabolome data;
- At: TwentyGreen (1 mo); training in specific microbiological analysis of prebiotics and get inside on how to develop customer information.
Enrolment in Doctoral degree:
ESR2 will be enrolled at the Department of Agricultural and Food Science (DISTAL) of University of Bologna.