Objective: Introduction in The part of the digestive system that consists of the stomach and intestines. ecology (microbiology, immunology, and host–pathogen interaction) and its influence on health and disease in young animals
Duration: 5 days
- Analytical tools in gut microbiology and immunology;
- definition of gut health;
- microbiota of pigs – What do we know and what do we aim at?;
- strategies for immunological defence of the The part of the digestive system that consists of the stomach and intestines.;
- evolution of immunological memory and the pig as a model for humans;
- gut microbiota in swine: composition, genetic parameters, and links with immunity and production traits;
- early-life nutrition – impact on gut function and immunity.
- Techniques for sampling and handling of porcine gut intestinal content;
- isolation and growth of gut bacteria;
- physiological and molecular characterisation of selected gut bacteria;
- analysis of microbial metabolites (short-chain fatty acids, gaseous compounds);
- genotypic characterisation of the gut microbiota;
- in vitro systems for studying gut biology (e.g. gut epithelium cell lines; isolation and characterisation of immune cells, including phenotyping by flow cytometry;
- enzyme- linked immunosorbent assay.