Objective: Introduction in GIT ecology (microbiology, immunology, and host–pathogen interaction) and its influence on health and disease in young animals
Duration: 5 days
- Analytical tools in gut microbiology and immunology;
- definition of gut health;
- microbiota of pigs – What do we know and what do we aim at?;
- strategies for immunological defence of the GIT;
- evolution of immunological memory and the pig as a model for humans;
- gut microbiota in swine: composition, genetic parameters, and links with immunity and production traits;
- early-life nutrition – impact on gut function and immunity.
- Techniques for sampling and handling of porcine gut intestinal content;
- isolation and growth of gut bacteria;
- physiological and molecular characterisation of selected gut bacteria;
- analysis of microbial metabolites (short-chain fatty acids, gaseous compounds);
- genotypic characterisation of the gut microbiota;
- in vitro systems for studying gut biology (e.g. gut epithelium cell lines; isolation and characterisation of immune cells, including phenotyping by flow cytometry;
- enzyme- linked immunosorbent assay.
HØJBERG[AU], DALGAARD[AU], CANIBE[AU], LAURIDSEN [AU], GARDINER [WIT], invited lectures from TREVISI [UNIBO].